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Author(s): Kanti Nage1, Dhananjay Tandon*2, Madhavi Tiwari3, Rupal Purena4, Khushbu Verma5, Prashanta Kumar Mitra6

Email(s): 1, 2dhananjay.25t@gmail.com, 3, 4, 5, 6

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    1Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492016, C.G., India
    2Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492016, C.G., India
    3Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492016, C.G., India
    4Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492016, C.G., India
    5Department of Applied Science, Shri Rawatpura Sarkar University, Raipur-492016, C.G., India
    6Department of Life Science, Shri Rawatpura Sarkar University, Raipur-492016, C.G., India
    *Corresponding Author Email- dhananjay.25t@gmail.com

Published In:   Volume - 8,      Issue - 1,     Year - 2026

DOI: 10.52228/NBW-JAAB.2026-8-1-2  

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ABSTRACT:
The women are at great risk of breast cancer. The mushrooms contain diverse chemicals with therapeutic properties. This study examined the cytotoxic effect of exo- and endo-polymers of Ganoderma sp., Pleurotus florida, Pleurotus eous, Hexagonia sp., and Schizophyllum sp. against the triple-negative breast cancer cell line (MDA-MB-231). The fungi were subjected for polymer production in batch culture. The supernatant was filtered and treated with ethanol. The treated supernatants were centrifuged and dried. Total reducing sugar, total carbohydrate, total polysaccharide, and total protein were assessed. The DPPH assay was conducted for the antioxidant assay. The MTT assay was conducted to evaluate the cytotoxic effects. The results are presented as percentage viability and percentage inhibition. The highest yield was found in GSENP. PEENP contained higher total carbohydrate. Total reducing sugars were higher in PEEXP, total polysaccharide was recorded highest in PEENP, while total protein was highest in SSENP. The highest inhibitions against the cell line were observed in GSENP with IC50 34.5±1.1 µg/ml. The GSENP exhibited a higher DPPH inhibition property with IC50 52.94±0.88 µg/ml. The results were statistically significant at p<0.001. The GSENP may good candidate in cancer treatment.

Cite this article:
Kanti Nage, Dhananjay Tandon, Madhavi Tiwari, Rupal Purena, Khushbu Verma, Prashanta Kumar Mitra (2026) Correlation between biochemical, antioxidant and anticancer activity of mushroom polymers. NewBioWorld A Journal of Alumni Association of Biotechnology, 8(1):15-35.DOI: https://doi.org/10.52228/NBW-JAAB.2026-8-1-2


Altun I, Sonkaya A (2018) The most common side effects experienced by patients were receiving first cycle of chemotherapy. Iran J Public Health, 47(8):1218-1219.

Arroyo-Cruz LV, Sagardía-González S, Miller K, Ling T, Rivas F, Martínez-Montemayor MM (2024) Selective antineoplastic potential of fractionated Caribbean native Ganoderma species extracts on triple-negative breast cancer cells. Pharmaceuticals (Basel), 17(7):864.

Aslam MS, Naveed S, Ahmed A, Abbas Z, Gull I, Athar MA (2014) Side effects of chemotherapy in cancer patients and evaluation of patients opinion about starvation based differential chemotherapy. J Cancer Ther, 5(8):817-822.

Banerjee S, Samajpati N, Samajpati P (1970) Revision of Bengal Polyporaceae: I. Genus Hexagonia. Indian J Mycol Res, 8(2):1-17.

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72(1-2):248-254.

Brand-Williams W, Cuvelier ME, Berset CL (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol, 28(1):25-30.

Bray F, Laversanne M, Sung H, Ferlay J, Siegel RL, Soerjomataram I, Jemal A (2024) Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 74(3):229-263.

Carr C, Ng J, Wigmore T (2008) The side effects of chemotherapeutic agents. Curr Anaesth Crit Care, 19(2):70-79.

Chandrawanshi NK, Tandia DK, Jadhav SK (2019) Determination of Anti-Diabetic Property of organic and nonorganic solvent extracts of Schizophyllum commune. NewBioWorld A Journal of Alumni Association of Biotechnology, 1(1):5-8.

Chen J, Seviour R (2007) Medicinal importance of fungal beta-(1→3),(1→6)-glucans. Mycol Res, 111(6):635-652.

Chen X, Sun-Waterhouse D, Yao W, Li X, Zhao M, You L (2021) Free radical-mediated degradation of polysaccharides: Mechanism of free radical formation and degradation, influence factors and product properties. Food Chem, 365:130524.

Chen Z, Yin C, Fan X, Ma K, Yao F, Zhou R, et al (2020) Characterization of physicochemical and biological properties of Schizophyllum commune polysaccharide extracted with different methods. Int J Biol Macromol, 156:1425-1434.

Cheng W, Yang Y, Zhang J, Di FQ, Li L, Wang C, et al (2023) Protective mechanisms of intra-and extracellular polysaccharides from Schizophyllum commune on H2O2-induced oxidative damage of human skin fibroblasts. Food Agric Immunol, 34(1):2203407.

Corso CR, Mulinari Turin de Oliveira N, Moura Cordeiro L, Sauruk da Silva K, da Silva Soczek SH, Frota Rossato V, et al (2021) Polysaccharides with antitumor effect in breast cancer: a systematic review of non-clinical studies. Nutrients, 13(6):2008.

Dasgupta D, Basu P, Paul A, Acharya K, Chakraborty N (2025) Schizophyllum commune, an underrated edible and medicinal mushroom: farm to industry. Stud Fungi, 10(1).

De Silva DD, Rapior S, Fons F, Bahkali AH, Hyde KD (2012) Medicinal mushrooms in supportive cancer therapies: an approach to anti-cancer effects and putative mechanisms of action. Fungal Divers, 55(1):1-35.

Demain AL, Vaishnav P (2011) Natural products for cancer chemotherapy. Microb Biotechnol, 4(6):687-699.

Gao X, Homayoonfal M (2023) Exploring the anti-cancer potential of Ganoderma lucidum polysaccharides (GLPs) and their versatile role in enhancing drug delivery systems: a multifaceted approach to combat cancer. Cancer Cell Int, 23(1):324.

Golisch B, Lei Z, Tamura K, Brumer H (2021) Configured for the human gut microbiota: molecular mechanisms of dietary $\beta$-glucan utilization. ACS Chem Biol, 16(11):2087-2102.

Gulcin İ (2020) Antioxidants and antioxidant methods: An updated overview. Arch Toxicol, 94(3):651-715.

Hanyu X, Lanyue L, Miao D, Wentao F, Cangran C, Hui S (2020) Effect of Ganoderma applanatum polysaccharides on MAPK/ERK pathway affecting autophagy in breast cancer MCF-7 cells. Int J Biol Macromol, 146:353-362.

Huang X, Nie S (2015) The structure of mushroom polysaccharides and their beneficial role in health. Food Funct, 6(10):3205-3217.

Jiao C, Qiu J, Gong C, Li X, Liang H, He C, et al (2025) Ganoderma lucidum extract reverses multidrug resistance in breast cancer cells through inhibiting ATPase activity of the P-glycoprotein via MAPK/ERK signaling pathway. Exp Cell Res, 444(2):114355.

Joseph S, Sabulal B, George V, Antony KR, Janardhanan KK (2011). Antitumor and anti-inflammatory activities of polysaccharides isolated from Ganoderma lucidum. Acta pharmaceutica, 61(3):335-42.

Kao C, Jesuthasan AC, Bishop KS, Glucina MP, Ferguson LR (2013) Anti-cancer activities of Ganoderma lucidum: active ingredients and pathways. Funct Foods Health Dis, 3(2):48-65.

Kaushal A (2020) Breast Cancer in Women, Signs and Treatment Approaches. NewBioWorld A Journal of Alumni Association of Biotechnology, 2(1):25-27

Kayl AE, Meyers CA (2006) Side-effects of chemotherapy and quality of life in ovarian and breast cancer patients. Curr Opin Obstet Gynecol, 18(1):24-28.

Kim HM, Paik SY, Ra KS, Koo KB, Yun JW, Choi JW (2006) Enhanced production of exopolysaccharides by fed-batch culture of Ganoderma resinaceum DG-6556. J Microbiol, 44(2):233-242.

Kleijburg FE, Wösten HA (2025) The versatility of Schizophyllum commune in nature and application. Fungal Biol Rev, 53:100431.

Klein DR (2020). Organic Chemistry. John Wiley & Sons.

Kumar R, Sharma S, Verma P, Singh J (2022) Schizophyllan: A bioactive polysaccharide from Schizophyllum commune and its therapeutic potential. J Fungi (Basel), 8(4):356.

Kuo HC, Liu YW, Lum CC, Hsu KD, Lin SP, Hsieh CW, et al (2021) Ganoderma formosanum exopolysaccharides inhibit tumor growth via immunomodulation. Int J Mol Sci, 22(20):11251.

Li C, Gao H, Yin C, Liu M, Fan X, Shi D, et al (2025) Physicochemical, antioxidant, gastrointestinal digestion and probiotic growth promoting properties of water and alkali extracted polysaccharides from Schizophyllum commune. J Sci Food Agric, 105(7):3787-3797.

Liu Z, Jiao Y, Lu H, Shu X, Chen Q (2020) Chemical characterization, antioxidant properties and anticancer activity of exopolysaccharides from Floccularia luteovirens. Carbohydr Polym, 229:115432.

Loyd AL, Barnes CW, Held BW, Schink MJ, Smith ME, Smith JA, et al (2018) Elucidating "lucidum": Distinguishing the diverse laccate Ganoderma species of the United States. PLoS One, 13(7):e0199738.

Mahajan M (2022) Schizophyllum commune. Emerg Infect Dis, 28(3):725.

Maiti S, Mallick S, Bhattacharya S (2011) Antitumor activities of bioactive proteins isolated from Pleurotus species. Int J Med Mushrooms, 13(2):1–10.

Mau JL, Lin HC, Chen CC (2002) Antioxidant properties of several medicinal mushrooms. J Agric Food Chem, 50(21):6072–6077.

Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem, 31(3):426–428.

Mirzayans R, Andrais B, Murray D (2018) Viability assessment following anticancer treatment requires single-cell visualization. Cancers (Basel), 10(8):255.

Mishra V, Tomar S, Yadav P, Singh MP (2021) Promising anticancer activity of polysaccharides and other macromolecules derived from oyster mushroom (Pleurotus sp.): An updated review. Int J Biol Macromol, 182:1628-1637.

Motulsky H, Christopoulos A (2004) Fitting models to biological data using linear and nonlinear regression: a practical guide to curve fitting. Oxford University Press, 27.

Mu S, Yang W, Huang G (2021) Antioxidant activities and mechanisms of polysaccharides. Chem Biol Drug Des, 97(3):628-632.

Muzaffar M, Rasool F, Sofi TA, Shikari AB, Mir SA, Lone GM (2023) Morphological characterization of different Pleurotus species under Kashmir conditions. Pharma Innovation, 12(7):3767-3770.

Naeem A, Hu P, Yang M, Zhang J, Liu Y, Zhu W, et al (2022) Natural products as anticancer agents: current status and future perspectives. Molecules, 27(23):8367.

Nandi S, Sikder R, Rapior S, Arnould S, Simal-Gandara J, Acharya K (2024) A review for cancer treatment with mushroom metabolites through targeting mitochondrial signaling pathway: In vitro and in vivo evaluations, clinical studies and future prospects for mycomedicine. Fitoterapia, 172:105681.

Nielsen SS (2024) Total carbohydrate by phenol-sulfuric acid method. In: Nielsen's Food Analysis Laboratory Manual, 147-151.

Ogidi CO, Ubaru AM, Ladi-Lawal T, Thonda OA, Aladejana OM, Malomo O (2020) Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues. Heliyon, 6(12):e05701.

Park HJ (2022) Current uses of mushrooms in cancer treatment and their anticancer mechanisms. Int J Mol Sci, 23(18):10502.

Partridge AH, Burstein HJ, Winer EP (2001) Side effects of chemotherapy and combined chemohormonal therapy in women with early-stage breast cancer. JNCI Monogr, 2001(30):135-142.

Plosca MP, Chiș MS, Fărcaș AC, Păucean A (2025) Ganoderma lucidum—From ancient remedies to modern applications: Chemistry, benefits, and safety. Antioxidants, 14(5):513.

Priyadarshini A (2018) Comparative analysis of morphology and taxonomy of different Pleurotus species found in Jharkhand. Int J Emerg Technol Innov Res, 5(8):752-754.

Ren D, Jiao Y, Yang X, Yuan L, Guo J, Zhao Y (2015) Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus. Chem Biol Interact, 237:166-174.

Ríos-Arrabal S, Artacho-Cordón F, León J, Román-Marinetto E, Salinas-Asensio MM, Calvente I, et al (2013) Involvement of free radicals in breast cancer. SpringerPlus, 2:404.

Sánchez C (2016) Reactive oxygen species and antioxidant properties from mushrooms. Synth Syst Biotechnol, 2(1):13–22.

Sánchez C (2017) Bioactives from mushroom and their application. In: Food Bioactives: Extraction and Biotechnology Applications, 23-57.

Sathishkumar K, Sankarapillai J, Mathew A, Nair RA, Gangane N, Khuraijam S, et al (2024) Breast cancer survival in India across 11 geographic areas under the National Cancer Registry Programme. Cancer, 130(10):1816-1825.

Sebaugh JL (2011) Guidelines for accurate EC50/IC50 estimation. Pharm Stat, 10(2):128-134.

Shafiq MA, Imene HH, Tasneem H, Muntaha ST, Almu MA, Nasir S (2025) The role of mushrooms and their bioactive compounds in cancer prevention and treatment. Prev Treat, 17:18.

Shang D, Li Y, Wang C, Wang X, Yu Z, Fu X (2011) A novel polysaccharide from Se-enriched Ganoderma lucidum induces apoptosis of human breast cancer cells. Oncol Rep, 25(1):267-272.

Sharma A, Sharma A, Tripathi A (2021) Biological activities of Pleurotus spp. polysaccharides: A review. J Food Biochem, 45(6):e13748.

Sipping M, Thakur D, Singh B, Njamen D, Mukherjee S (2025) Polysaccharide extract of Ganoderma resinaceum restricts breast cancer progression in vitro by intervening in epithelial–mesenchymal transition and cancer stemness. Rev Bras Farmacogn, 35(6):1404-1424.

Sun LX, Li WD, Lin ZB, Duan XS, Xing EH, Jiang MM, Yang N, Qi HH, Sun Y, Li M, Niu YD. Cytokine production suppression by culture supernatant of B16F10 cells and amelioration by Ganoderma lucidum polysaccharides in activated lymphocytes (2015). Cell and Tissue Research, 360(2):379-89.

Talib WH, Alsayed AR, Barakat M, Abu-Taha MI, Mahmod AI (2021) Targeting drug chemo-resistance in cancer using natural products. Biomedicines, 9(10):1353.

Thang TD, Kuo PC, Ngoc NTB, Hwang TL, Yang ML, Ta SH, et al (2015) Chemical constituents from the fruiting bodies of Hexagonia apiaria and their anti-inflammatory activity. J Nat Prod, 78(11):2552-2558.

Thulasi EP, Thomas PD, Ravichandran B, Madhusudhanan K (2010) Mycelial culture and spawn production of two oyster mushrooms, Pleurotus florida and Pleurotus eous on different substrates. Int J Biol Technol, 1(3):39-42.

Tirkey S, Verma S, Dhiver T, Shukla K (2025) Species-Specific Variation in Nutrients and Antioxidant Activity among Calocybe Mushrooms. NewBioWorld A Journal of Alumni Association of Biotechnology, 7(1):19-26.

Uddin Pk MM, Pervin R, Biswas MS, Rahman MM (2022) An antioxidant polysaccharide from Ganoderma lucidum induces apoptotic activity in breast cancer cell line. bioRxiv [Preprint], doi:10.1101/2022.01.

Ukaegbu CI, Shah SR, Hamid HA, Normaiza Z, Alara OR (2019) Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation. J Food Meas Charact, 13(1):368-382.

Vadnerker P, Vyas T, Kapadia C (2022) Characterization of exopolysaccharide produced by Ganoderma sp. TP and its immunomodulatory properties. Rom Biotechnol Lett, 28:3527-3535.

Wan WAAQI, Latif NA, Harvey LM, McNeil B (2016) Production of exopolysaccharide by Ganoderma lucidum in a repeated-batch fermentation. Biocatal Agric Biotechnol, 6:91-101.

Wang J, Hu S, Nie S, Yu Q, Xie M (2016) Reviews on mechanisms of in vitro antioxidant activity of polysaccharides. Oxid Med Cell Longev, 2016:5692852.

Zhang QY, Wang FX, Jia KK, Kong LD (2018) Natural product interventions for chemotherapy and radiotherapy-induced side effects. Front Pharmacol, 9:1253.

Zhao T, Yang M, Ma L, Liu X, Ding Q, Chai G, et al (2023) Structural modification and biological activity of polysaccharides. Molecules, 28(14):5416. 

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