Abstract View

Author(s): Harsha Verma11, 22, *3

Email(s): 1vermaharsha465@gmail.com

Address:

    1Govt. Nagarjuna PG College of Science, Raipur, Chhattisgarh, India
    22School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    *Corresponding Author Email- vermaharsha465@gmail.com

Published In:   Volume - 2,      Issue - 2,     Year - 2020

DOI: 10.52228/NBW-JAAB.2020-2-2-5  

 View HTML        View PDF

Please allow Pop-Up for this website to view PDF file.

ABSTRACT:
Fluorescence In-Situ Hybridization (FISH) introduced in the 1980s, is a technique useful in gene mapping, detection of various types of abnormalities, such as fusion of genes and genomic fusion and deletions. Gradual implemented improvements have led to more precise patient diagnostics along with matching the best therapies. This technique has been now especially used in the field of cancer diagnostics and treatment. Some excellent examples discussed below include genetic tests like ALK and ROS1 gene performed in lung cancer.

Cite this article:
Harsha Verma (2020) Use of Fluorescence In-Situ Hybridization (FISH) in Diagnosis of Cancer. NewBioWorld A Journal of Alumni Association of Biotechnology, 2(2):18-20.DOI: https://doi.org/10.52228/NBW-JAAB.2020-2-2-5


Asif A, Mushtaq S, Hassan U, Akhtar N, Hussain M, Azam M, Qazi R (2018) Fluorescence in Situ Hybridization (FISH) for Differential Diagnosis of Soft Tissue Sarcomas. Asian Pac. J. Cancer Prev. APJCP, 19:655–660.

Huang WE, Stoecker K, Griffiths R, Newbold L, Daims H, Whiteley AS, Wagner M (2007) Raman FISH combining stable‐isotope raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function. Environmental Microbiology, 9(8):1878–1889.

Jordan R, Edington J, Evans HH, Schwartz JL (1999) Detection of chromosome aberrations by FISH as a function of cell division cycle (harlequin-FISH). Biotechniques, 26:532-34.

Mertens F, Antonescu CR, Mitelman F (2016) Gene fusions in soft tissue tumors: recurrent and overlapping pathogenetic themes, The Journal of Pathology Clinical Research, 55: 291– 310.

O'connor C (2008) Fluorescence in situ hybridization (FISH). Nature Education, 1:171.

Salido M, Pijuan L, Martínez-Avilés L, Galván A.B, Cañadas I, Rovira A, Zanui M, Martínez A (2011) Increased ALK Gene Copy Number and Amplification Are Frequent in Non-Small Cell Lung Cancer. J. Thorac. Oncol. Off. Publ. Int. Assoc. Study Lung Cancer, 6:21–27. 

Slamon DJ, Godolphin W, Jones LA, Holt JA, Wong SG, Keith DE, Levin WJ, Stuart SG, Udove J, Ullrich A (1989) Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science, 244:707-12.

Zwirglmaier K, Fichtl K, Ludwig W (2005) In situ functional gene analysis: recognition of individual genes by fluorescence in situ hybridization. Methods Enzymol., 397:338–51.


Related Images:



Recent Images



Therapeutic Use of Some Common Medicinal Plants for the Treatment of Major Life Style Diseases of Chhattisgarh
Butterflies and Their Conservation Status on the Campus of Sant Guru Ghasidas Government P.G. College Kurud, Chhattisgarh, India
Fermentation of Tomato Juice with S. cerevisiae has enhanced nutrition and shelf-life
Review on double haploid in rice plant (Oryza sativa L.)
A  Review of Clinical Aspect of Dhatura: According to Ayurveda
An aphid transmitted banana bunchy top disease of banana and its detection: A Review
Comparative Evaluation of In Vitro Antimicrobial Efficacy of different Species of Curcuma against Human Pathogenic Bacteria
Phytoremediation: A Sustainable Approach to Combat Heavy Metal Contaminated Soil - A Review
A brief overview of plant abiotic stresses
Formulation and Evaluation of Herbal Toothpaste against Biofilm Producing Staphylococcus aureus

Tags


Recomonded Articles: