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Author(s): Naveen Dewangan*1, Khemraj Sahu2, Harish Bhardwaj3

Email(s): 1naveendkumardew2@gmail.com, 2khemrajsahu1197@gamil.com, 3harishbhardwaj808@gmail.com

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    1School of Studies of Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    2School of Studies of Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    33 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    *Corresponding Author Email- naveendkumardew2@gmail.com

Published In:   Volume - 2,      Issue - 1,     Year - 2020

DOI: 10.52228/NBW-JAAB.2020-2-1-3  

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ABSTRACT:
Polymerase Chain Reaction (PCR) has revolutionized the field of molecular biology since its invention in 1983. The technique has enabled scientists to amplify specific sequences of the genomic DNA for various applications, from basic research to clinical diagnosis. However, like any other experimental protocol, PCR has its limitations and challenges. Troubleshooting and optimization are crucial steps for achieving consistent and reliable results in PCR. This review provides an overview of the history of PCR, highlighting its evolution over the past four decades. The article also discusses common troubleshooting strategies and optimization methods that researchers use to overcome obstacles in PCR experiments. By understanding the principles and techniques of PCR optimization, scientists can design experiments that efficiently amplify specific DNA sequences, minimize non-specific amplification, and improve the overall accuracy and sensitivity of the results.

Cite this article:
Naveen Dewangan, Khemraj Sahu, Harish Bhardwaj (2020) Revolutionizing Molecular Biology: The Evolution of PCR through Troubleshooting and Optimization. NewBioWorld A Journal of Alumni Association of Biotechnology, 2(1):1-17.DOI: https://doi.org/10.52228/NBW-JAAB.2020-2-1-3


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