The phytochemical constituents, quantitative antioxidant, and total phenolic content of Hardwicikia binata Roxb. plant parts were investigated in the present study. Methanolic extracts of H. binata leaves and stems were obtained using the traditional extraction methods, soxhlet and maceration. Preliminary tests for phytochemicals, revealed the presence of flavonoid, steroids, tannin and terpenoids, tannins in the stem and, flavonoids and phenols in the methanolic extracts of the stem and leaves, respectively. Saponin was found in the leaves and stem parts, but no alkaloids were identified in either. The high antioxidant content of the H. binata stem and leaf methanolic extract was proven by the total antioxidant assay. Furthermore, total phenol quantification among the extracts revealed that the plant's stem extract was rich in phenols. DPPH scavenging activity showed the potential antioxidant capability of H. binata parts. Overall, the preliminary findings revealed the bioactive components and antioxidant properties of H. binata leaves and stem parts; further detailed examination and screening of the plant may prove it to be a potential source of medication in the pharmaceutical field.
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