Abstract View

Author(s): Afreen Anjum1, Afaque Quraishi*2

Email(s): 1afrinanjum301@gmail.com, 2drafaque13@gmail.com


    1School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, INDIA
    2School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, INDIA
    *Corresponding Author Email- drafaque13@gmail.com

Published In:   Volume - 4,      Issue - 1,     Year - 2022

DOI: 10.52228/NBW-JAAB.2022-4-1-2  

 View HTML        View PDF

Please allow Pop-Up for this website to view PDF file.

Curcuma caesia Roxb., also known as black turmeric, is a rare rhizomatous herb in the Zingiberaceae family. In the present study, we used the two different sucrose concentrations (3% and 9% sucrose) in Murashige and Skoog medium supplemented with 8 mg/l benzyladenine, 8 mg/l kinetin, 100 mg/l citric acid, 200 mg/l adenine sulfate and 2 mg/l indole-3-acetic acid to investigate the biochemical differences in the leaves of C. caesia. Biochemical parameters such as total sugar, proline, protein, chlorophyll, and hydrogen peroxide (H2O2) content, as well as superoxide dismutase (SOD) and catalase (CAT) activity, were analyzed to study the differences. All the parameters were performed on the 0th and 30th day after inoculation (DAI). It has been found that as sucrose concentration raised from 3% to 9% sucrose, the SOD activity, as well as the total sugar, proline, protein, chlorophyll, and H2O2 content increases significantly at 30th DAI. These increased enzymatic and non-enzymatic defense systems, thus helping the plant to maintain its growth under a stressed environment.

Cite this article:
Afreen Anjum, Afaque Quraishi (2022) Investigation of biochemical changes in the leaves of Curcuma caesia Roxb. under sucrose-induced osmotic stress environment. NewBioWorld A Journal of Alumni Association of Biotechnology,4(1):1-6.DOI: https://doi.org/10.52228/NBW-JAAB.2022-4-1-2


Ahmad MSA, Javed F, Ashraf M (2007) Iso-osmotic effect of NaCl and PEG on growth, cations and free proline accumulation in callus tissue of two indica rice (Oryza sativa L.) genotypes. Plant Growth Regulation, 53:53-63.


Anjum A, Singh V, Adil S, Quraishi A (2022) In Vitro Propagation of Curcuma caesia Roxb. via Bud Culture Technique and ISSR Profiling of the Plantlets for Genetic Homogeneity. Research Journal of Biotechnology (Accepted).


Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1):205-207.


Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72:248-254.


Chance B, Maehly AC (1959) The assay of catalase and peroxidase. In: Click D (ed) Methods of Biochemical Analysis, Vol 1 Interscience Publishers, New York.


Chang CC, Locy RD, Smeda R, Sahi SV, Singh NK (1997) Photoautotrophic tobacco cells adapted to grow at high salinity. Plant Cell Reports, 16:495-502.


Chauhan R, Keshavkant S, Quraishi A (2018) Enhanced production of diosgenin through elicitation in micro-tubers of Chlorophytum borivilianum Sant et Fernand. Industrial Crops and Products, 113:234-239.


Devi HP, Mazumder PB, Devi LP (2015) Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts. Toxicology report, 2:423-428.


Dhindsa RS, Dhindsa PP, Thorpe TA (1981) Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32:93-101.


Dubois M, Gilles KA, Hamilton JK, Roberts PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical chemistry28(3):350-356.

Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48:909-930.


Haida Z, Sinniah UR, Nakasha JJ, Hakiman M (2022) Shoot Induction, Multiplication, Rooting and Acclimatization of Black Turmeric (Curcuma caesia Roxb.): An Important and Endangered Curcuma Species. Horticulturae, 8(8):740.


Hiscox JDT, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57(12):1332-1334.


Ikeda T, Fujime Y, Terabayashi S, Date S (2002) Water status of garlic callus under various salt and osmotic stress conditions. Horticultural Science, 37:404-405.


Javed F (2002c) In vitro salt tolerance in wheat. III: Water relations in callus. International Journal of Agriculture and Biology, 4:465-467.


Javed F, Ikram S (2008) Effect of sucrose induced osmotic stress on callus growth and biochemical aspects of two wheat genotypes. Pakistan Journal of Botany, 40(4):1487-1495.


Karmakar I, Dolai N, Saha P, Sarkar N, Bala A, Haldar P K (2011) Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Oriental Pharmacy and Experimental Medicine, 11:221-228.


Kim SH, Kim SK (2002) Effect of sucrose level and nitrogen source on fresh weight and anthocyanin production in cell suspention culture of ‘Sheridan’ Grape (Vitis spp). Journal of Plant Biotechnology, 4:2327-2330.


Kolarovic  L, Valentovic P, Luxova M, Gasparıkova O (2009) Changes in antioxidants and cell damage in heterotrophic maize seedlings differing in drought sensitivity after exposure to short-term osmotic stress. Plant Growth Reregulation, 59:21-26.


Krishnan PN, Decruse SW, Radha RK (2011) Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology. In Vitro Cellular and Developmental Biology-Plant, 47(1):110-122.


Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiologia Plantarum, 8:100-127.


Luna CM, Pastori GM, Driscoll S, Groten K, Bernard S, Foyer CH (2004) Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. Journal of Experimental Botany, 56:417-23.


Mahato D, Sharma HP (2017) Kali Haldi, an ethnomedicinal plant of Jharkhand state- A Review. Indian Journal of Traditional Knowledge, 17(2):322-326.


Mehta UJ, Krishnamurthy VK, Hazra S (2000) Regeneration of plant via adventitious bud formation from zygotic embryo axis of tamarind (Tamarindus indica). Current Science, 78: 1231-12.


Mishra M (2013) Conservation of critically endangered medicinal plant Curcuma caesia in the natural forests of Mandla district, Madhya Pradesh.  A Journal of Environment and Biodiversity, 4(3):36-40.


Moharramnejad S, Sofalian O, Valizadeh M, Agari A, Shiri M (2016) Response of antioxidant defense system to osmotic stress in maize seedlings. Fresenius Environmental Bulletin, 25:805-811.


Munnik T, Meijer HJ (2001) Osmotic stress activates distinct lipid and MAPK signalling pathways in plants. FEBS Letters, 498:172-178.


Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15:473-497.


Noman A,  Ali Q, Naseem J, Javed MT, Kanwal H, Islam W, Aqeel M, Khalid N, Zafar S, Tayyeb M,  Iqbal N,  Buriro M, Maqsood J, Shahid S (2018) Sugar beet extract acts as a natural bio-stimulant for physio-biochemical attributes in water stressed wheat (Triticum aestivum L.) Acta Physiologiae Plantarum, 40(6):1-7.


Sarma I, Deka AC, Sarma TC (2021) A Protocol for Rapid Clonal Propagation and Microrhizome Production of Curcuma caesia Roxb. (Zingiberaceae): A Critically Endangerd Medicinal Plant of North East India. Indian Journal of Agricultural Research, 55(1):13-22.

Sharma N, Verma PP, Murthy SN (2017) Pharmacognostical evaluation and conservation of threatened species Curcuma caesia Roxb. International Journal of Ayurvedic Medicine, 8(2):68-72.


Stewart CR, Morris CJ, Thompson JF (1966) Changes in Amino Acid Content of Excised Leaves During Incubation II. Role of Sugar in the Accumulation of Proline in Wilted Leaves. Plant Physiology, 41:1585-1590.


Swedlund B, Locy RD (1993) Sorbitol as the primary carbon source for the growth of embryogenic callus of maize. Plant Physiology, 103:1339-1346.


Valenzuelaa XG, Moyab EG, Cruza QR, Estrellac LH, Santacruza GAA (2005) Chlorophyll accumulation is enhanced by osmotic stress in graminaceous chlorophyllic cells. Journal of Plant Physiology, 162:650-661.


Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant system in acid rain-treated bean plants, protect role of exogenous polyamines. Plant Sciience, 151:59-66.


Verma D, Srivastava S, Singh V, Rawat AKS (2010) Pharmacognostic evaluation of Curcuma caesia Roxb. rhizome. Natural Product Sciences, 16(2):107-110.


Wang CQ, Li RC (2008) Enhancement of superoxide dismutase activity in the leaves of white clover (Trifolium repens L.) in response to polyethylene glycol-induced water stress. Acta Physiological Plantarum, 30:841-847.


Winicov I, Button JD (1991) Accumulation of photosynthesis gene transcripts in response to sodium chloride by salt-tolerant alfalfa cells. Planta, 183:478-483.


Zhang J, Jia W, Yang J, Ismail AM (2006) Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Research, 97:111-119.


Related Images:

Recent Images

Therapeutic Use of Some Common Medicinal Plants for the Treatment of Major Life Style Diseases of Chhattisgarh
Butterflies and Their Conservation Status on the Campus of Sant Guru Ghasidas Government P.G. College Kurud, Chhattisgarh, India
Fermentation of Tomato Juice with S. cerevisiae has enhanced nutrition and shelf-life
Review on double haploid in rice plant (Oryza sativa L.)
A  Review of Clinical Aspect of Dhatura: According to Ayurveda
An aphid transmitted banana bunchy top disease of banana and its detection: A Review
Comparative Evaluation of In Vitro Antimicrobial Efficacy of different Species of Curcuma against Human Pathogenic Bacteria
Phytoremediation: A Sustainable Approach to Combat Heavy Metal Contaminated Soil - A Review
A brief overview of plant abiotic stresses
Formulation and Evaluation of Herbal Toothpaste against Biofilm Producing Staphylococcus aureus


Recomonded Articles:

Author(s): Jipsi Chandra; Apurva Mishra; S. Keshavkant

DOI: 10.52228/NBW-JAAB.2020-2-2-2         Access: Open Access Read More

Author(s): Krishna Kumar Verma; Shristi Soni; Pratiksha Pandey; Varsha Sahu; Sumit Kumar Dubey; Neha Behar*

DOI: 10.52228/NBW-JAAB.2023-5-1-4         Access: Open Access Read More