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Author(s): Apurva Singh1, Dristi Verma2, Shubhra Tiwari*3, S.K. Jadhav4

Email(s): 1, 2, 3shubhratiwari77@gmail.com, 4jadhav9862@gmail.com

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    1MATS University, Raipur, Chhattisgarh, India
    2School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    3School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    4School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
    *Corresponding Author Email- shubhratiwari77@gmail.com

Published In:   Volume - 3,      Issue - 1,     Year - 2021

DOI: 10.52228/NBW-JAAB.2021-3-1-4  

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ABSTRACT:
Infectious diseases produced by bacteria are an urgent healthcare concern worldwide. Several infections in humans are caused by bacterial agents such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Staphylococcus aureus. The current development of resistance to antibiotics and accompanying toxicity issues is causing a rise in studies into the antibacterial role of plants against resistant strains due to relative effectiveness and safety. The current work was done to check the antibacterial activity of Azadirachta indica leaves (Neem) along with the green synthesis of silver nanoparticles. Methanol and Acetone extracts of Neem leaves were tested against Klebsiella oxytoca and Staphylococcus aureus, both of which are resistant to antibiotics. Silver nanoparticle synthesized in methanol was very effective against Klebsiella oxytoca (13.5mm).

Cite this article:
Apurva Singh, Dristi Verma, Shubhra Tiwari, S.K. Jadhav (2021) Antimicrobial activity of Azadirachta indica (Neem) leaf extract on gram positive and gram-negative bacteria. NewBioWorld A Journal of Alumni Association of Biotechnology, 3(1):11-17.DOI: https://doi.org/10.52228/NBW-JAAB.2021-3-1-4


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Alzohairy MA (2016) Therapeutics role of Azadirachta indica (Neem) and their active constituents in diseases prevention and treatment. Evidence-Based Complementary and Alternative Medicine2016.

Banerjee P, Satapathy M, Mukhopahayay A, Das P (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property, and toxicity analysis. Bioresources and Bioprocessing, 1:1-10.

Lalitha A, Subbaiya R, Ponmurugan P (2013) Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. International Journal of Current Microbiology and Applied Sciences, 2:228-235.

Maleki L, Sadeghian-Rizi T, Ghannadian M, Sanati MH, Shafizadegan S, Sadeghi-Aliabadi, H (2017) Antibacterial activity of Azadirachta indica leaf extracts against some pathogenic standards and clinical bacterial isolates. Avicenna Journal of Clinical Microbiology and Infection, 5:12987-12987.

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Rautela A, Rani J (2019). Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms. Journal of Analytical Science and Technology, 10:1-10.

Roy P, Das B, Mohanty A, Mohapatra S (2017) Green synthesis of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study. Applied Nanoscience, 7:843-850.

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