Abstract View

Author(s): Vikram Singh1, Afaque Quraishi2

Email(s): 1dr_afaque@rediffmail.com

Address:

    1School of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur 492 010, India
    2School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India
    *Corresponding author

Published In:   Volume - 1,      Issue - 1,     Year - 2019

DOI: 10.52228/NBW-JAAB.2019-1-1-5  

 View HTML        View PDF

Please allow Pop-Up for this website to view PDF file.

ABSTRACT:
For producing reliable and reproducible results via Polymerase chain reaction (PCR) for applications like molecular diagnostics for plant pathogens and plant molecular biology requires good quality DNA. One of the disadvantages of using traditional methods for DNA extraction and storage is the utilization of expensive reagents and equipments. The current study attempts for rapid and reliable method for DNA extraction and detection of Banana bunchy top virus (BBTV) within banana material using InstantDNA cards. This is the first successful application of InstantDNA card for extraction of banana viruses, particularly for BBTV.

Cite this article:
Vikram Singh and Afaque Quraishi (2019) Use of InstantDNA card for molecular detection of Banana bunchy top virus by PCR in Musa sp. NewBioWorld A Journal of Alumni Association of Biotechnology, 1(1): 16-18.DOI: https://doi.org/10.52228/NBW-JAAB.2019-1-1-5


References

Chi MH, Park SY, Lee YH (2009) A Quick and Safe Method for Fungal DNA Extraction. Plant Pathology Journal, 25:108-111.

FAOStat (2014) FAO production statistics for banana and plantain 2012. Food and Agriculture Organization, Rome. http://faostat.fao.org/

Guo JR, Schnieder F, Abd-Elsalam KA, Verreet JA (2005) Rapid and efficient extraction of genomic DNA from different phytopathogenic fungi using DNAzol reagent. Biotechnology Letter, 27:3-6.

Liu D, Coloe S, Baird R, Pedersen J (2000) Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology, 38:471.

Mbogori MN, Kimani M, Kuria A, Lagat M, Danson JW (2006) Optimization of FTA technology for large scale plant DNA isolation for use in marker assisted selection. African Journal of Biotechnology, 5:693-696.

Punyarani K, Devi KD, Singh CH, Singh NS, Singh HH, Singh TD, Moirangthem S, Devi HS (2013) In vitro production of genetically stable and virus free plantlets of Musa sp. var. Meitei Hei using male inflorescence as explant. Scientia Horticulturae, 164:440-447.

Qazi J (2016) Banana bunchy top virus and the bunchy top disease. Journal of General Plant Pathology, 82:2-11.

Safarpour M, Sinniah UR, Subramaniam S, Swamy MK (2017) A novel technique for Musa acuminata Colla ‘Grand Naine’ (AAA) micropropagation through transverse sectioning of the shoot apex. In Vitro Cellular Developmental Biology-Plant, 53:226-238.

Selvarajan R, Balasubramanian V, Sasireka T (2015) A simple, rapid and solvent free nucleic acid extraction protocol for detection of banana bunchy top virus by polymerase chain reaction and loop-mediated isothermal amplification. European Journal of Plant Pathology, 142:389-396.

Selvarajan R, Sheeba M, Balasubramanian V, Rajmohan R, Lakshmi DN, Sasireka T (2010) Molecular characterization of geographically different Banana Bunchy Top Virus (BBTV) isolates in India. Indian Journal of Virology, 21:110-116.

Sharma K, Bhattacharjee R, Sartie A, Kumar PL (2013) An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction. African Journal of Biotechnology, 12:1894-1901.

Siegel CS, Stevenson FO, Zimmer EA (2017) Evaluation and comparison of FTA card and CTAB DNA extraction methods for non-agricultural taxa. Applications in Plant Sciences, doi:10.3732/apps.1600109.

Singh V, Koche V, Quraishi A (2018) viral elimination strategies for Musa spp. Research & ReviewsA Journal of Microbiology and Virology8:7-14. 

Thomson D, Henry R (1995) Single-step protocol for preparation of plant-tissue for analysis by PCR. Biotechniques, 19:394-400.

Watanabe S, Greenwell AM, Bressan A (2013) Localization, concentration, and transmission efficiency of banana bunchy top virus in four asexual lineages of Pentalonia aphids. Viruses, 5:758-775.

Related Images:



Recent Images



Therapeutic Use of Some Common Medicinal Plants for the Treatment of Major Life Style Diseases of Chhattisgarh
Butterflies and Their Conservation Status on the Campus of Sant Guru Ghasidas Government P.G. College Kurud, Chhattisgarh, India
Fermentation of Tomato Juice with S. cerevisiae has enhanced nutrition and shelf-life
Review on double haploid in rice plant (Oryza sativa L.)
A  Review of Clinical Aspect of Dhatura: According to Ayurveda
An aphid transmitted banana bunchy top disease of banana and its detection: A Review
Comparative Evaluation of In Vitro Antimicrobial Efficacy of different Species of Curcuma against Human Pathogenic Bacteria
Phytoremediation: A Sustainable Approach to Combat Heavy Metal Contaminated Soil - A Review
A brief overview of plant abiotic stresses
Formulation and Evaluation of Herbal Toothpaste against Biofilm Producing Staphylococcus aureus

Tags


Recomonded Articles: