For producing reliable and reproducible results via Polymerase chain reaction (PCR) for applications like molecular diagnostics for plant pathogens and plant molecular biology requires good quality DNA. One of the disadvantages of using traditional methods for DNA extraction and storage is the utilization of expensive reagents and equipments. The current study attempts for rapid and reliable method for DNA extraction and detection of Banana bunchy top virus (BBTV) within banana material using InstantDNA cards. This is the first successful application of InstantDNA card for extraction of banana viruses, particularly for BBTV.
Cite this article:
Vikram Singh and Afaque Quraishi (2019) Use of InstantDNA card for molecular detection of Banana bunchy top virus by PCR in Musa sp. NewBioWorld A Journal of Alumni Association of Biotechnology, 1(1): 16-18.DOI: https://doi.org/10.52228/NBW-JAAB.2019-1-1-5
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